Protocols for macroalgae research / Bénédicte Charrier, Thomas Wichard, and CRK Reddy.

Includes bibliographical references.

Section I: Cultivating and preserving seaweeds -- Chapter 1 Seaweed in high-energy environments: Protocol to move Saccharina cultivation offshore / Bela H. Buck and Britta Grote -- Chapter 2 Cultivation protocol for Saccharina latissimi / Silje Forbord, Kristine Braaten Steinhovden, Kaia Kjølbo Rød, Aleksander Handå, and Jorunn Skjermo -- Chapter 3 Derivation of clonal stock cultures and hybridization of kelps: A tool for strain preservation and breeding programs / Inka Bartsch -- Chapter 4 Cryopreservation of macroalgae / John G. Day -- Chapter 5 Unraveling seaweeds bacteriomes: From field site to computer screen / Tânia Aires, Gerard Muyzer, Ester A. Serrão, and Aschwin H. Engelen -- Chapter 6 Heavy metal ecotoxicity on the early life history stages of macroalgae / Pablo P. Leal and Michael Y. Roleda -- Chapter 7 A simple protocol for a rapid and consistent production of a large number of viable protoplasts from the Ulvophycean species / Vishal Gupta and C.R.K. Reddy -- Chapter 8 Purification of sporulation and swarming inhibitors from Ulva: Application in algal life-cycle controlling / Ralf W. Kessler, Taghreed Alsufyani, and Thomas Wichard -- Chapter 9 Preparation of axenic cultures in Ulva (Chlorophyta) / Gianmaria Califano and Thomas Wichard -- Section II: Chemical composition -- Chapter 10 Biochar production from seaweeds / Loretto Contreras-Porcia, Matías Araya, Elizabeth Garrido-Ramírez, Cristian Bulboa, Jean Pierre Remonsellez, Javier Zapata, Camila Espinoza, and Jorge Rivas -- Chapter 11 Identification and quantification of laminarins in brown algae / Angelika Graiff, Wolfgang Ruth, and Ulf Karsten -- Chapter 12 Determination of carbohydrate composition of macroalgae / Wouter J.J. Huijgen, E.M. Cobussen-Pool, B.F. van Egmond, and J.W. van Hal -- Chapter 13 Quantification of proteins in seaweeds / Carl Safi, Jelle van Leeuwen, Yvette Telleman, Nicole Engelen-Smit, Lambertus van den Broek, and Paulien Harmsen -- Chapter 14 Comprehensive phytohormone quantification in the red alga Pyropia yezoensis by liquid chromatography–mass spectrometry / Takakazu Matsuura, Izumi C. Mori, Yoko Ikeda, Takashi Hirayama, and Koji Mikami -- Chapter 15 Total phenolic content and antioxidant capacity analysis of seaweed biomass / Xiaoru Hou, Randi Neerup, and Anne-Belinda Bjerre -- Chapter 16 Extraction of phycocyanin and phycoerythrin pigments / Stewart William Beattie, Michèle Morançais, Paul Déléris, Joël Fleurence, and Justine Dumay -- Chapter 17 Quantification and localization of reactive oxygen species in marine macrophytes / Manoj Kumar, Loretto Contreras-Porcia, Nirali M. Kumar, and Peter J. Ralph -- Chapter 18 Metabolomics of intra- and extracellular metabolites from micro- and macroalgae using GC–MS and LC–MS Constanze Kuhlisch, Gianmaria Califano, Thomas Wichard, and Georg Pohnert -- Chapter 19 Preparative extraction of exometabolites from seaweed surfaces / Florian Weinberger -- Chapter 20 Disruption-free solid-phase extraction of surface metabolites from macroalgae / Emilio Cirri and Georg Pohnert -- Section III: Cellular and molecular characterization -- Chapter 21 The immunodetection and in situ imaging of cell-wall polysaccharides in brown algae / Amandine Siméon, Delphine Duffieux, Cécile Hervé, Sophie Le Panse, Paul Knox, and Thomas Torode -- Chapter 22 Atomic force microscopy based analysis of cell-wall elasticity in macroalgae / Thomas Torode, Marina Linardic, J. Louis Kaplan, and Siobhan A. Braybrook -- Chapter 23 Dynamic and microscale mapping of cell growth: Case of Ectocarpus filament cells / Hervé Rabillé, Bernard Billoud, Elodie Rolland, and Bénédicte Charrier -- Chapter 24 Actin fluorescent staining in the filamentous brown alga Ectocarpus siliculosus / Hervé Rabillé, Maria Koutalianou, Bénédicte Charrier, and Christos Katsaros -- Chapter 25 Cryofixation of brown algae for transmission electron microscopy / Chikako Nagasato, Christos Katsaros, and Taizo Motomura -- Chapter 26 Probing the subcellular topography of seaweeds: Transmission electron microscopy, immunocytochemistry, and correlative light microscopy / Sandra C. Raimundo and David S. Domozych -- Chapter 27 Coralline algae preparation for scanning electron microscopy and optical microscopy / S. Kaleb, G. Alongi, and A. Falace -- Chapter 28 Extraction of high quality RNA from brown algae for transcriptomic analysis / Sandra Heinrich -- Chapter 29 Induction of sexual reproduction in Spirogyra cultures for laser capture microdissection of gametes and zygotes / Denis Saint-Marcoux and Jane A. Langdale -- Chapter 30 Cloning and expression strategies for the postgenomic analysis of brown algae / Agnès Groisillier -- Chapter 31 Polyethylene glycol-mediated transformation in the green macroalga Ulva mutabilis (Chlorophyta): A forward genetics approach / Jens Boesger, Michiel Kwantes, and Thomas Wichard – Index.

Protocols for Macroalgae Research describes state-of-the-art approaches and applications in seaweed research. The book is comprised of 31 protocols in all and provides critical information regarding the cultivation and preservation of seaweeds, their chemical composition, as well as their cellular and molecular characterization. This type of research is critical and timely, as the last few decades have witnessed remarkable advancements in phycological research worldwide. Consideration of algal resources for food, fuel, chemicals, feed, and fertilizer has furthered the development of diverse laboratory techniques that not only advance their utilization substantially, but also provided new insights in understanding the molecular basis of their form and function. This recent progress has enabled researchers to determine and quantify various metabolites and organic macromolecules such as carbohydrates, proteins, and lipids.

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